Note: This known issue was fixed in CMS 6.5 rev. 110419 (Technical Preview) for the setup.exe. This known issue still applies when you install Sitecore from the ZIP file.
Sitecore might fail to start with the following errors when you run the application in an ASP.NET 4.0 application pool using Classic pipeline mode.
The page you are requesting cannot be served because of the ISAPI and CGI Restriction list settings on the Web server.
The requested content appears to be script and will not be served by the static file handler.
This happens if the IIS configuration does not allow the .NET 4 aspnet_isapi.dll extension to execute.